DNA
Part:BBa_J119013
Designed by: Caleb J. Carr Group: Eckdahl Lab (2011-08-18)
FINISH
Finish half edge for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. BsmBI site pointing right that when digested frees up half edge word "GTGG" on the 5' end that allows ligation to any gene 2 half-edge. Followed by the BsaI site pointing left that when digested frees up the FINISH word "CGAG" that allows ligation to the new tr5f4HPP vector. In between these two sites is an 8 nt spacer. This section is flanked by EcoRI and PstI sticky ends for cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 30
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Categories
Parameters
None |